STUDY ON OXYGEN SUPPLY CONDITION AND INFLUENCE OF ALGAL IN PARTIAL NITRIFICATION PROCESS IN A BACTERIA AND ALGAE SYMBIOTIC SYSTEM
In this study,the optimal partial nitrification(PN)process was achieved by adjusting the light intensity and algae concentration,and their effects on effluent quality,dissolved oxygen(DO)concentration,the number and enzyme activity of ammonia oxidizing bacteria(AOB),the content of algae photosynthetic pigments,microbial morphology and community structure were investigated.The results of water quality measurement showed that the nitrite accumulation rate reached 88.88％under the condition of 16000 Lux illumination intensity and 1170 mL Chlorella culture solution(OD680=1.6±0.4),achieving a good PN effect.The DO monitoring results showed that the reactor was always in the concentration range of 0.1～0.11 mg/L,and the algae concentration was the main factor affecting the DO concentration in the reactor.The ammonia monooxygenase(AMO)activity and amoA gene copy number of AOB bacteria,and the concentration of three photosynthetic pigments of algae gradually stabilized at the later stage of reactor operation,indicating that AOB bacteria and algae gradually formed a stable symbiotic system in the reactor.High-throughput sequencing results showed that Nitrosomonas and Chlorella were the main functional microorganisms,and the morphologies of two functional microorganisms were observed by scanning electron microscopy.In this paper,the effect of different algal oxygen supply conditions on the activity,quantity of functional microorganisms and microbial community structure in the PN process were investigated,which provided a theoretical reference for regulating algal oxygen supply during the start-up and stable operation of the process.
bacterial-algal symbiosis systempartial nitrificationdissolved oxygenchlorophyllmicrobial community
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