RAPID START-UP AND MICROBIAL COMMUNITY ANALYSIS OF A SULFUR AUTOTROPHIC DENITRIFICATION COUPLED ANAEROBIC AMMONIA OXIDATION DENITRIFICATION SYSTEM
In this paper,the sulfur autotrophic denitrification(SAD)process start-up was accomplished in an SBR reactor using sodium thiosulfate as an electron donor,and a gradual increase in substrate loading.The rapid start-up and stable operation of the sulfur autotrophic denitrification coupled with anaerobic ammonia oxidation(SAD/A)autotrophic denitrification system was developed by inoculating Anammox sludge with a combined pH and NO3--N/NH4+-N regulation strategy.The results showed that in 30 days,the denitrification sludge had been domesticated into SAD sludge.After 90 days,the SBR reactor had been successfully enriched with SAD sludge,and more than 85％of NO3--N had been removed from the system.The SAD/A system was successfully started after 14 days of operation with an optimal inoculation ratio of 3∶1 for both two species of sludge.After 30 days,the NO3--N removal rate of the system could reach 85％and the NH4+-N removal rate could reach 80％.The coupled system's best denitrification efficiency was achieved at a pH of 8 and a NO3--N/NH4+-N ratio of 1.8,and it operated steadily.High-throughput sequencing results showed that the overall species diversity and richness in the SAD/A system was increased after coupling,and the dominant microbial genera were Candidatus Brocadia and Thiobacillus,with similar relative abundances,indicating a good coupling effect of SAD/A.
Anammoxsulfur autotrophic denitrificationSAD/ANO2--N accumulationmicrobial community structure
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