SCREENING OF LIPID-DEGRADING BACTERIA AND OPTIMIZATION OF ENZYME-PRODUCING CONDITION FOR PERISHABLE KITCHEN ORGANIC WASTE
In recent years,the production of perishable organic waste in kitchens has been increasing.To improve the degradation rate,it is urgent to develop an efficient process for degrading perishable organic waste in the kitchen.In this paper,we screened a bacterial strain that can adapt to perishable organic waste in the kitchen and degrade oil efficiently.Samples were collected from perishable organic waste and oil-contaminated soil,and 16S rDNA sequencing technology was used to construct a strain growth tree,and response surface analysis was used to study the optimal growth conditions of the strain.Through screening and functional verification,a lipid-degrading strain,named as TH1,was obtained and identified as Serratia marcescens.The single-factor experiment and response surface method showed that the lipase activity reached 213.70 U/L under the condition of temperature of 30 ℃,pH=7.54,and salinity of 1％.The results can provide germplasm resources for the subsequent development of composite bacterioides for efficient degradation,mechanism research,and resource utilization of perishable kitchen organic waste.
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