好氧颗粒污泥强化脱氮及微生物群落的演变
ENHANCED NITROGEN REMOVAL BY AEROBIC GRANULAR SLUDGE AND EVOLUTION OF MICROBIAL COMMUNITY
赵勇 1郝桂珍 2徐利 2熊晓莹 3张暄茉 3王佳伟2
作者信息
- 1. 河套学院 水利与土木工程系,内蒙古巴彦淖尔 015000
- 2. 河北建筑工程学院 市政与环境工程系,河北张家口 075000;河北省水质工程与水资源综合利用重点实验室,河北张家口 075000
- 3. 河北建筑工程学院 市政与环境工程系,河北张家口 075000
- 折叠
摘要
为实现好氧颗粒污泥(AGS)系统高效的脱氮效果,在AGS系统前端设置厌氧段形成"厌氧/好氧"(A/O)运行模式来强化AGS系统的脱氮效果;同时研究整个试验过程中AGS的污泥特性和微生物群落结构的变化.结果表明:AGS以丝状菌作为骨架,活性污泥在水力剪切力的作用下不断地黏附在骨架上,进而形成AGS;其过程中胞外聚合物(EPS)组分中蛋白质含量显著增加,污泥表面Zeta电位值降低,提高了污泥间的凝聚性能,保证了 AGS的形成和稳定性运行.污泥颗粒化阶段,unclassified_f_Sphingomonadaceae、unclassified_o_Saccharimonadales 等具有疏水性且分泌EPS功能菌属的相对丰度上升,促进了污泥颗粒化.A/0运行模式的介入提高了 AGS系统中与脱氮相关菌属的相对丰度,强化了 AGS系统的脱氮性能;第110天低温环境的介入下,AGS系统依然保持稳定运行,最终TN去除率上升至 78.2％,其主要归功于 Zoogloea(由 4.6％升至 8.2％)、Thauera(由 2.0％升至 2.3％)、Rhodobacter(由 2.3％升至2.7％)、norank_f_Actinomycetaceae(由 1.8％升至 3.7％)、Pseudoxanthomonas(由 2.2％升至 4.5％)等菌属的相对丰度上升.
Abstract
To realize the efficient nitrogen removal of aerobic granular sludge(AGS),this study set an anaerobic section at the front end of the AGS system to form an anaerobic/aerobic(A/O)operation mode,to enhance the denitrification of the AGS system.At the same time,the changes in sludge characteristics and microbial community structure of AGS during the whole experiment were studied.The results showed that AGS was composed of filamentous bacteria as the skeleton,and the activated sludge continuously adhered to the skeleton under the action of hydraulic shear force to form AGS.During the process,the protein content in the extracellular polymeric substance(EPS)increased significantly,and the Zeta potential value of the sludge surface decreased,which improved the coagulation performance between the sludge and ensured the formation and stable operation of AGS.In the sludge granulation stage,the relative abundance of hydrophobic and EPS-secreting bacteria,such as unclassified_f_Sphingomonadaceae and unclassified_o_Saccharimonadales increased,which promoted sludge granulation.The intervention of A/O operation mode increased the relative abundance of nitrogen-related bacteria in the AGS system and enhanced the nitrogen removal performance of the AGS system.On the 110th day,the interventional AGS system in the low-temperature environment still maintained stable operation,and the final TN removal rate increased to 78.2％.The main reason was attributed to the increase in the relative abundance of Zoogloea(4.6％→8.2％),Thauera(2.0％→ 2.3％),Rhodobacter(2.3％→ 2.7％),norank_f_Actinomycetaceae(1.8％→ 3.7％),Pseudoxanthomonas(2.2％→4.5％),etc.
关键词
好氧颗粒污泥/微生物群落/强化脱氮/A/O模式Key words
aerobic granular sludge/microbial community/strengthen denitrification/A/O model引用本文复制引用
基金项目
河北省高等学校科学技术研究项目(ZD2021316)
河北省研究生创新资助项目(CXZZSS2022062)
出版年
2024
NETL
NSTL
万方数据