CONSTRUCTION OF A SHORTCUT NITROGEN REMOVAL SYSTEM FOR ALGAL-BACTERIAL SYMBIOSIS AND ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE IN SLUDGE
The conventional biological nitrogen removal of wastewater with low C/N ratio and high ammonia nitrogen has some deficiencies,such as high energy consumption of aeration,and high amount of added carbon source.In this study,a shortcut nitrogen removal system for algal-bacterial symbiosis was constructed by combining shortcut nitrification functional sludge enriched with ammonia-oxidizing bacteria(AOB)with Chlorella.The effects of the film coating method and light intensity on nitrogen and phosphorus removal in the system were studied.The high-throughput sequencing technology was used to analyze the microbial community structure,and to analyze and determine the nitrogen conversion pathway in the system.The results demonstrated that the relative abundance of AOB in acclimated sludge was 19.31％,of which the main strain was Nitrosomonas,accounting for 94.41％of total AOB.The shortcut nitrification ability of acclimated sludge was excellent.An efficient nitrogen removal was achieved in the algal-bacterial symbiotic system.When first inoculating sludge and then inoculating microalgae,and the light intensity was set to 5000 lux,the best removal efficiency of TN and TP from simulated biogas slurry in the system could be realized as high as 93.22％and 82.38％,respectively.After stable operation of the system for 37 days,the microbial richness of the algal-bacterial symbiotic biofilm increased,and the nitrogen removal efficiency was higher than 90％.In biofilm,Thauera,with an relative abundance of 56.42％,played a dominant role in denitrification,while the relative abundance of AOB was reduced to 5.65％,and the abundance of NOB was very low.The high efficiency of nitrogen removal was realized through the shortcut nitrification-denitrification process(about 88.46％)and biological assimilation(about 6.79％)in shortcut nitrogen removal system for algal-bacterial symbiosis.About 60.5％of the carbon source for denitrification was saved,compared with traditional biological denitrification technology.
algal-bacterial symbiosissystem constructionmicrobial community structurenitrogen conversionshortcut nitrification-denitrification
万方数据
维普
