To investigate the feasibility of the mixture design methodology applied to the construction of composite inoculum,and the optimization of components to address the current problems of long composting cycles and low wood fiber conversion efficiency of garden waste,the degrading strains from the GW compost samples and their interrelationships were identified,using isolation culture and antagonism experiments.The non-antagonistic strains were mixed in different proportions using the mixture design method and their enzyme activities were measured,after which the mathematical models between the proportions of different strains and the enzyme activities were established.The results showed the composite consisted of Bacillus tequilensis,Bacillus velezensis,and Bacillus oryzaecorticis,and their optimum ratio was 28.95％:39.63％:31.42％.Meanwhile,in the degradation experiment using GW as the carbon source,the maximum enzyme activity of laccase was 47.25 U/L,lignin peroxidase was 66.67 U/L,manganese peroxidase was 99.65 U/L,carboxymethyl cellulase was 90.66 U/mL,and filter paper activity was 48.44 U/mL.The degradation rates of cellulose and lignin on the 18th day could reach 13.48％and 9.73％,respectively.The composite inoculum constructed based on the mixture design showed superior short-term enzyme activity production and biodegradation capacity,which is conducive to the application of GW composting.
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