EFFECT OF ADDING METHANOL ON MICROBIAL COMMUNITIES AND ARGs IN A WASTEWATER TREATMENT PLANT OF AN INDUSTRIAL PARK
Methanol is widely used as a carbon source to improve the efficiency of wastewater treatment with a low carbon to nitrogen ratio.However,the influences of methanol addition on treatment efficiency of the water treatment facilities in the industrial parks are still rarely reported and unsufficiently understood,especially for the changes in antibiotic resistance genes(ARGs).Here,high-throughput sequencing and metagenomic sequencing methods were employed to reveal the treatment effects using oxidation ditch+MBR process with methanol addition,and the changes in the relative abundance of functional microorganisms and ARGs in a waste water treatment plant of an industrial park in Xinjiang.The results showed that methanol addition improved the effluent quality to consistently meet the class A of the Discharge Standard of Pollutants for Municipal Wastewater Treatment Plants(GB 18918-2002).Furthermore,we found that the relative abundance of unclassified_c_Gammaproteobacteria with methyl degrading function,and Hyphomicrobium with denitrifying function in activated sludge was improved to 31.84％and 28.68％,respectively.More importantly,the total ARGs in the influent were 150.77× 10-6,but decreased to 25.77× 10-6 in the effluent,indicating that methanol addition significantly reduced ARGs.In summary,these results shed light on understanding the roles of methanol addition in the wastewater treatment process,and further provide data support and theoretical guidance for the controlling of new pollutants ARGs in the industrial parks.
万方数据
维普
