The efficient utilization of biomass energy from a wide range of sources is beneficial to achieving the goal of sustainable green energy.In this study,the dark light mixed fermentation technology was used to enrich four kinds of dark light mixed bacterial flora from aerobic sludge,anaerobic sludge,lake mud and cow dung,and the anaerobic fermentation of glucose was used to produce hydrogen.In this study,microorganisms,hydrogen production,growth,and material change characteristics during fermentation were investigated.The 16S rRNA high-throughput results showed that all four flora were dark-light mixed colonies dominated by the genus Rhodopseudomonas sp.,with a diversity of flora structures,and obvious differences among the four flora.Among the four dark-light hybrid flora,the hydrogen production performance of the cow dung hybrid flora was the best,with a cumulative hydrogen yield of(633.93±4.87)mL/L,a maximum hydrogen production rate of(8.55±0.31)mL/(L·h),and an energy conversion rate of 9.22％,with 7.32％of the electrons in the substrate flowed to the hydrogen,under the condition of 30 mmol/L glucose,35 ℃,and 5000 lux.The results showed that the dark-light hybrid flora could efficiently utilize the substrate for hydrogen production by anaerobic fermentation.
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