Determination of 18 Cephalosporin Antibiotic Residues in Surface Water by Automatic Solid Phase Extraction-Ultra Performance Liquid Chromatography Tandem Mass Spectrometry
In this study,an analytical method of ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)was established,which can simultaneously determine the residues of 18 cephalosporins in surface water.The automatic solid phase extraction technology is used for pretreatment.Surface water samples are enriched by HLB solid phase extraction column and eluted with 1.0％(V/V)formic acid methanol solution.BEH Shield RP18(1.7 μm,2.1 mm × 100 mm)chromatographic column is preferred to be used for separation.It can be ionized in the positive mode of electrospray(ESI+),be detected in multiple reaction mode(MRM)and measured by external standard method.The results showed that the linear relationship of 18 cephalosporin antibiotics is good in the concentration range of 5.0～100 μg/L,the correlation coefficients(r)are greater than 0.999,the detection limit of the method is in the range of 0.5～1.8 ng/L,and the quantitative limit is in the range of 2.0～7.2 ng/L.Standard addition recovery experiments on surface water samples showed that the recoveries of cephalosporins are 64.3％～92.8％with the relative standard deviations in the range of 0.5％～14.5％.With high level of automation and stable and reliable results,the current method is suitable for the determination of cephalosporins in surface water.
Cephalosporin antibioticsAutomatic solid-phase extractionUltra-high performance liquid chromatography tandem mass spectrometrySurface water
万方数据
维普
