Assessing Insect Biodiversity Under Different Forest Stands in Nanjing Based on Environmental DNA Metabarcoding Techniques
To investigate the feasibility of DNA metabarcoding technology in insect biodiversity surveys under different forest types,this study employed Malaise traps from March 2022 to May 2022 to conduct insect biodiversity surveys in artificial forests,mixed forests,and secondary forests in Luhe District,Gaochun District,and Jiangning District of Nanjing city,Jiangsu Province.Excluding data from typical aquatic insect orders such as Lepidoptera and Odonata,the eDNA metabarcoding technology annotated 11 orders,100 families,330 species,and 465 OTUs with 521,219 sequences of terrestrial insects,among which Lepidoptera,Diptera,and Hemiptera were the dominant groups.The Chao1 and ACE indices showed that species richness was highest in secondary forests,followed by mixed forests and artificial forests.The Shannon diversity index indicated that secondary forests had relatively good biodiversity,followed by mixed forests,while artificial forests had relatively low biodiversity.Based on this,we discuss the performance of environmental DNA metabarcoding technology in monitoring terrestrial insect diversity,aiming to provide technical reference for new methods of monitoring terrestrial insect biodiversity.
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