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冬季野鸭湖湿地植物根际微生物群落结构分析

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文章应用磷脂脂肪酸(PLFA)技术研究野鸭湖湿地扁秆蔗草、芦苇和茭白根际土壤微生物的群落结构,同时分析了根际微生物与微生物量磷的相关关系.结果表明,扁秆蔗草根际微生物量最高,达60 nmol/g,其次为茭白和芦苇.3种植物根际土壤中的优势菌是以16∶0、18∶0、i16∶0为代表的好氧菌;根际土壤中也出现以18∶2ω6,9、18∶1ω9c为代表的真菌.影响冬季扁秆蔗草、茭白根际微生物量及群落结构的主要因素分别是枯落物和气温,而芦苇的特殊根系使其在冬季有较好的适应性.植物根际微生物含量与土壤微生物量磷相关性较好,其中细菌含量与微生物量磷呈显著正相关关系.因此,根际微生物受植物种类影响较大,建议寒冷地区人工湿地根据扁秆藤草、芦苇、茭白的不同特点交错种植,且植物进入衰亡期进行收割有利于污染物去除.
Study on Rhizosphere Microbial Community Structure of Plants at Yeyahu Wetland in Winter by PLFA Analysis
This paper introduced the technique of phospholipid fatty acid (PLFA) which was used to study the microbial community structure in rhizosphere soil of Scirpus planiculmis,Phragmites communis and Zizania aquatica in Yeyahu Wetland,the relationship between microbial community structure and microbial biomass phosphorus was also analyzed.The study showed that rhizosphere microbial biomass of Scirpus planiculmis was the highest,amounting to 60 nmol/g,and followed by Zizania aquatic and Phragmites communis.The aerobic bacteria 16∶0,18∶0 and i16∶0 were typical of the predominant bacteria in rhizosphere soil and 18∶2ω6,9,18 ∶1ω9c were also the typical of the fungi.The main factors affecting microbial biomass and community structure in rhizosphere of Scirpus planiculmis and Zizania aquatic were temperature and litter,but Phragmites communis had a better adaptability because of its special root.There was a good correlation between microbial biomass and phosphorus in microbial biomass,and bacteria content and microbial biomass phosphorus content were significantly positively correlated.Therefore,the wetland plant rhizosphere microorganisms were influenced by species of the plant.According to the characteristics of different plants,it is recommended that planting and harvesting in a staggered way in the northern frigid regions and harvesting in plant decay period,which would be helpful to pollutants removal.

wetland plantsmicrobial community structurerhizosphere soilphospholipid fatty acids

朱芸芸、李敏、赵暾、曲博、其美

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北京林业大学环境科学与工程学院,北京100083

湿地植物 微生物群落结构 根际土壤 磷脂脂肪酸

国家自然科学基金人事部留学基金委择优资助项目

51279004优秀项目类

2016

环境科学与技术
湖北省环境科学研究院

环境科学与技术

CSTPCDCSCD北大核心
影响因子:0.943
ISSN:1003-6504
年,卷(期):2016.39(9)
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