环境科学与技术2024，Vol.47Issue(2) ：35-44.DOI:10.19672/j.cnki.1003-6504.1720.23.338

宁夏第三排水沟底泥氮循环细菌群落结构特征

Characteristics of Nitrogen Cycling Bacterial Community Structure in Sediments of the Third Drainage Ditch in Ningxia

郑兰香 赵靖 黄凌霄
环境科学与技术2024，Vol.47Issue(2) ：35-44.DOI:10.19672/j.cnki.1003-6504.1720.23.338
✕
  • 维普
  • 万方数据

宁夏第三排水沟底泥氮循环细菌群落结构特征

Characteristics of Nitrogen Cycling Bacterial Community Structure in Sediments of the Third Drainage Ditch in Ningxia

郑兰香 1赵靖 2黄凌霄3
扫码查看

作者信息

  • 1. 宁夏大学生态环境学院,宁夏 银川 750021;宁夏大学土木与水利工程学院,宁夏 银川 750021;宁夏回族自治区黄河水联网数字治水重点实验室,宁夏 银川 750021
  • 2. 宁夏大学土木与水利工程学院,宁夏 银川 750021;宁夏回族自治区黄河水联网数字治水重点实验室,宁夏 银川 750021
  • 3. 宁夏大学信息工程学院,宁夏 银川 750021
  • 折叠

摘要

底泥是水体氮循环过程的重要场所.为揭示宁夏第三排水沟底泥氮循环细菌群落结构特征,该文在5个采样点底泥理化性质分析基础上,采用荧光定量PCR法测定硝化和反硝化功能基因丰度,并对细菌群落与环境因子进行相关性分析.研究结果表明:除三排起点底泥中pH值为6.96以外,其余点均呈弱碱性.有机碳、铵态氮、硝态氮、全氮和全磷沿沟的空间分布均呈先降低后增高趋势.在硝化菌群落中,AOB amoA基因丰度(3.16×106～7.95×107 copies/g)远高于 AOA amoA(5.62×103～3.76×105 copies/g);反硝化菌群落中,nirS基因丰度(6.87×107～9.16×108 copies/g)高于nirK(2.40×107～3.42×108 copies/g).泉古菌门是AOA主要优势菌门,变形菌门为AOB、nirK和nirS共有的主要优势菌门.底泥中的氮循环微生物群落结构与环境因素的Spearman相关性分析表明,AOA与铵态氮呈显著正相关(P＜0.05),与TOC呈极显著负相关(P＜0.001);AOB与pH呈显著正相关;nirS型反硝化菌与pH呈显著负相关;nirK型反硝化菌与TOC呈极显著负相关,三排底泥氮循环功能微生物群落差别较大且受理化因子影响显著.

Abstract

Sediments are important sites of nitrogen cycling.To explore the characteristics of the bacterial community struc-ture involved in nitrogen cycling in the sediments of the Third Drainage Ditch in Ningxia,based on analysis of the physico-chemical properties of sediments at five sampling points,fluorescent quantitative PCR was used to determine the abundance of functional genes related to nitrification and denitrification,and correlation analysis was performed between the bacterial community and environmental factors.The results showed that except for the sediment at the starting point of the Third Drain-age Ditch with a pH value of 6.96,the sediments at other points exhibited weak alkalinity.Organic carbon,ammonium nitro-gen,nitrate nitrogen,total nitrogen,and total phosphorus showed a spatial distribution trend of decreasing and then increasing along the ditch.The abundance of AOB amoA genes(3.16×106～7.95×107 copies/g)in nitrifying bacteria was significantly higher than that of AOA amoA genes(5.62 ×103～3.76×105 copies/g).Concerning denitrifying bacteria,the abundance of nirS genes(6.87×107～9.16×108 copies/g)exceeded that of nirK genes(2.40×107～3.42×108 copies/g).The phylum Thaumarchaeota is the predominant group of ammonia-oxidizing archaea(AOA),while the phylum Proteobacteria is the major group consist-ing of both ammonia-oxidizing bacteria(AOB)as well as bacteria possessing nirK and nirS genes.The spearman correlation analysis between the nitrogen cycling microbial community structure in the sediments and environmental factors indicated a significant positive correlation between AOA and ammonium nitrogen(P＜0.05),as well as an extremely significant negative correlation between AOA and TOC(P＜0.001).AOB exhibited a significant positive correlation with pH,while nirS-type denitrifying bacteria showed a significant negative correlation with pH,and nirK-type denitrifying bacteria showed an extremely significant negative correlation with TOC.The results indicated significant differences in the bacterial community structure involved in nitrogen cycling in the sediments of the Third Drainage Ditch,and these were significantly influenced by physicochemical factors.

关键词

底泥/氮循环/群落结构/AOA/AOB/nirK/nirS

Key words

sediment/nitrogen cycling/bacterial community structure/AOA/AOB/nirK/nirS

引用本文复制引用

基金项目

国家自然科学基金(42267011)

宁夏回族自治区自然科学基金(2021AAC03096)

出版年

2024
环境科学与技术
湖北省环境科学研究院

环境科学与技术

CSTPCD北大核心
影响因子：0.943
ISSN：1003-6504
参考文献量20
段落导航相关论文