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巢湖不同入湖口微生物群落结构差异性分析

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微生物是湖泊生态环境的重要组成部分，驱动水生态系统的养分循环与能量转换。微生物因对环境因子变化高度敏感而常作为湖泊生态系统演化的指示生物，因此，研究巢湖不同入湖口微生物群落结构差异及其与环境因子的关系具有重要意义。该文采用基于16S rRNA基因的高通量测序技术分析巢湖不同入湖口微生物群落结构，利用随机矩阵原理构建优势微生物的共线网络探究不同入湖口微生物种间关系差异;利用多元线性回归(MRM)与曼特尔检验(Mantel Test)分析揭示不同入湖口微生物群落的驱动因子及其对环境变化的响应。结果表明:南淝河入湖口与派河入湖口微生物群落相似程度较高，与杭埠河入湖口微生物群落结构差异明显;3个入湖口共有优势微生物均与硝化反硝化作用相关，南淝河入湖口特有优势微生物为Flavobacteriaceae、Steroidobactera-ceae、Sphingomonadaceae 与 Sutterellaceae，派河入湖 口独有优势微生物为 Rhodanobacteraceae 与 Hydrogenophilaceae，杭埠河入湖口独有优势微生物为V icinamibacteraceae与Methylomonadaceae;微生物共线网络结果表明南淝河入湖口拥有共生关系最高的微生物网络，不同入湖口微生物网络中关键物种均与氮转化功能相关;MRM分析表明不同入湖口微生物群落结构的驱动因子存在差异，南淝河入湖口关键驱动因子为温度与NO3--N含量，派河入湖口关键驱动因子为水体NH4+-N含量，杭埠河入湖口关键驱动因子为温度、TP和COD含量;Mantel Test分析表明不同入湖口受驱动因子影响的微生物均为相对丰度差异较大的优势微生物。入湖口环境差异导致微生物群落组成与结构产生一定差异，营养程度较高的入湖口微生物共生关系更加明显，环境对微生物存在选择作用来维持生态环境的稳定。
Differential Analysis on Microbial Community Structure in Different River Inlets of Chaohu Lake
Microorganisms are a vital part of lake ecosystems,which drive nutrient cycling and energy conversion in aquatic ecosystems,and on the other hand are highly sensitive to changes of environmental factors,being oftentimes used as indicator organisms for the evolution of lake ecosystems.As for Chaohu Lake,it is of great significance to study the differenc-es in microbial community structure at different lake inlets and their relationship with environmental factors.In this study,the high-throughput sequencing technology based on 16S rRNA gene was used to analyze the structure of microbial communi-ties in different inlets of the Lake,and a covariate network of dominant microorganisms was constructed by adopting the principle of stochastic matrix to investigate the differences in inter-species relationships among microorganisms in different inlets,and then MRM and Mantel Test analyses were used to reveal the driving factors of the microbial communities and their response strategies to the environmental changes.The analysis by methods of MRM(multiple linear regression)and Man-tel Test revealed the driving factors of different microbial communities in the lake inlets and their responsive strategies to cope with the environmental changes.Findings of the study showed that microbial communities in inlets of into-lake rivers of the Nanfei River and Paihe River were of a rather good similarity,and the microbial community structure of the into-lake river Hangbu River inlet was obviously different,and the common dominant microorganisms of these three inlets were related to nitrification and denitrification,and the microorganisms of the three inlets are all related to the function of nitrification and de-nitrification;the dominant microorganisms endemic in the Nanfei River inlet included Flavobacteriaceae,Steroidobacteraceae,Sphingomonadaceae and Sutterellaceae,the unique dominant microorganisms in the inlet of the Paihe River was Rhodanobac-teraceae and Hydrogenophilaceae,and the unique dominant microorganisms in the Hangbu River inlet were Vicinamibactera-ceae and Methylomonadaceae.The results of the microbial co-linear network suggested that the Nanfei River inlet had the highest symbiotic microbial network,and the key species in the microbial network of different inlets were related to the nitro-gen transformation function;the MRM analysis showed that there existed differences in the driving factors of microbial com-munity structure in different lake inlets;the key driving factors in the inlet of the Nanfei River were water temperature and the content of NO3--N,the key driving factor in the inlet of the Paihe River was the content of NH4+-N,and the key driving fac-tors in the inlet of the Hangbu River were temperature,TP,and COD content;and the Mantel Test analysis indicated that all of the microorganisms in different lakes were dominant microorganisms with large differences in relative abundance,which were affected by the driving factors.Mantel Test analysis showed that the microorganisms affected by the driving factors in differ-ent lake inlets were dominant microorganisms with greater differences in relative abundance.In conclusion,the environmental differences in the lake inlets resulted in certain differences in terms of the composition and structure of microbial communi-ties;the microbial symbiosis was more distinct in the inlets with higher nutrient levels;and the environment naturally had a se-lective effect on the microorganisms to maintain the stability of the ecological environment.

Chaohu Lake inletmicroorganismsenvironmental factorsspatial differences

黄健、周俊杰、张华、李虎、罗涛、王金花、何春华、刘俊

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安徽建筑大学环境与能源工程学院,安徽 合肥 230601

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工业园区污染治理与资源化利用安徽省联合共建学科重点实验室,安徽 合肥 230041

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巢湖入湖口 微生物 环境因子 空间差异

安徽省重点研究与开发计划项目安徽省高校省级自然科学研究项目安徽省高校省级自然科学研究项目安徽省高校省级自然科学研究项目安徽建筑大学引进人才及博士启动基金项目安徽建筑大学引进人才及博士启动基金项目安徽省中青年教师培养行动项目

2023t070100022022AH010019KJ2021A06192022AH0502362019QDZ642019QDZ60DTR2023029

2024

10.19672/j.cnki.1003-6504.2464.23.338

环境科学与技术
湖北省环境科学研究院

环境科学与技术

CSTPCD北大核心
影响因子：0.943
ISSN：1003-6504
年,卷(期)：2024.47(3)