Cell-free Biosensor Based on Allosteric Transcription Factors for Detecting Arsenic Ions in Aquatic Environment
Aiming at developing a novel method for on-site quick detection of arsenic ions in aquatic environment,in this study a cell-free biosensor was designed using ArsR,an allosteric transcription factor that can specifically recognize arsenic,as recognition element with fluorescent RNA as output signal to realize the in-situ detection of arsenic ions.The results obtained with regard to the cell-free biosensor showed that under the optimal experimental condition,the developed biosensor could achieve the detection of 1.0 nmol/L arsenic ions within one hour,and quantitative detection in a linear dynamic range of 1～1 000 nmol/L was achieved with the assistance of a microplate reader,the linear equation being Y=1232lg[As3+]-629.5(R2=0.989 3).The result of arsenic ion detection using the biosensor basically consistented with the re-sult by use of atomic absorption spectroscopy in the conditon of measuring the water samples of river water,the recovery rate being in the range of 98.866％～102.001％;furthermore,the target arsenic ions could be detected correctly despite the inter-ference of other heavy metal ions.In summary,this method being characterized with high sensitivity and specificity certainly provided a new idea for the rapid on-site detection of arsenic ions.
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维普
