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高原强太阳辐射下A2O工艺活性污泥脱氮机制研究

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文章通过横向运行太阳辐射、恒温和室温3套平行A2O装置,分析高原太阳辐射对A2O反应器驯化活性污泥过程中微生物丰度和多样性及代谢的影响。结果表明,当运行到第40天时,太阳辐射A2O反应器的脱氮效果相比于恒温、室温装置总体上较差;太阳辐射A2O系统中的操作分类单元数量为595,呈下降趋势,驯化过程中优势菌属代谢功能以有机物降解、反硝化脱氮为主,代谢途径以碳氮代谢为主;反硝化菌的相对丰度随太阳辐射照射时长的增加而增加。运行到40d太阳辐射A2O系统菌群多样性要低于恒温和室温系统,某些常见的污泥中的优势菌属相对丰度受到了太阳辐射的抑制,但如norank_f_AKYH767脱氮相关的菌属丰度及其功能基因反而在太阳辐射反应器中得到了强化。
Study on the Mechanism of Activated Sludge Denitrification by A2O Process Under Strong Solar Radiation in Plateau
The effects of plateau solar radiation on microbial abundance and diversity and metabolism during the do-mestication of activated sludge in an A2O reactor were analyzed by running three parallel A2O units with solar radia-tion,constant temperature,and room temperature in a lateral manner.The results showed that when running to the 40th day,the denitrification effect of the solar radiation A2O reactor was generally worse compared with the constant temper-ature and room temperature units;the number of operational taxonomic units in the solar radiation A2O system was 595,which showed a decreasing trend,and the metabolic functions of the dominant genus of microorganisms were based on the degradation of organic matter,denitrification and denitrification,and the metabolic pathways were based on the carbon and nitrogen metabolism,and it was also found that the relative abundance and diversity of denitrifying bacteria relative abundance increased with the increase of the duration of solar radiation exposure.The bacterial diversi-ty of the solar-radiated A2O system was lower than that of the constant-and room-temperature systems up to 40 days of operation,and the relative abundance of some common dominant genera in sludge was suppressed by solar radiation,but the abundance of some denitrification-related genera and their functional genes were instead enhanced in the solar-radiated reactors.

plateauA2O processsolar radiationmicrobial community characterizationmetabolic pathwaysgene functions

彭贤湃、宗永臣、王俊、傅椿惠、纪家奥、张东艳

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西藏农牧学院水利土木工程学院,西藏 林芝 860000

西藏农牧学院西藏土木水利电力工程技术研究中心,西藏 林芝 860000

高原 A2O工艺 太阳辐射 微生物群落特征 代谢途径 基因功能

西藏自治区自然基金重点项目西藏农牧学院研究生创新计划资助项目

XZ202301ZR0056GYJS2024-46

2024

环境科学与技术
湖北省环境科学研究院

环境科学与技术

CSTPCD北大核心
影响因子:0.943
ISSN:1003-6504
年,卷(期):2024.47(4)