To investigate the aggregation state changes that occur when nanoparticles enter the environment and interact with inorganic minerals,a laboratory experiment was conducted with two types of layered double hydroxides,namely MgAl-LDH20 and MgAl-LDH90,were prepared at respectively 20 ℃ and 90 ℃,meantime the aggregation kinetics of both single and mixed nanoparticle of fullerene-C60 were studied using Zeta potential(ζ)analysis techniques.Consequently,the experi-ment showed that the critical aggregation concentration(CCC)values of MgAl-LDH20 and MgAl-LDH90 were 209.12 mmol/L and 166.27 mmol/L,respectively;the Zeta potential(ζ)of MgAl-LDHs suspension was significantly de-creased after being mixed with C60 suspension at different ratios(120∶1,60∶1,30∶1),and the decline degree of ζ increased with the raising of C60 concentration;and thekfastof MgAl-LDH was<3 in the single system,while thekfastof the mixed system was>3 after being added with C60,which suggested that the aggregation of the particles was accelerated due to the electrostatic interactions between MgAl-LDHs and C60,and the presence of MgAl-LDHs could slow down the mobility of C60 in the aqueous environment.Moreover,the stability of the mixed suspension system was determined by MgAl-LDHs when only a few amounts of C60 entered the LDHs suspension system.