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碳源小球藻强化人工湿地处理养猪沼液的效能与机制

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针对传统人工湿地处理畜禽养殖沼液反硝化不足、脱氮效果不佳的难题,该研究将小球藻(Chlorella sorokiniana)作为人工湿地外加碳源,构建小球藻强化人工湿地脱氮系统,通过添加不同浓度的小球藻(0、0。25、0。5、1。0g/L)来研究系统对养猪沼液水质的去除效果及溶解性有机物(DOMs)的变化规律,并从微生物角度解释强化脱氮机制。结果表明:(1)与未添加小球藻的人工湿地相比,总氮的去除率明显增高,最高可达70。24%,硝氮去除率最高达93。45%;(2)基于三维荧光平行因子分析,DOMs中3组分分别为富里酸/类腐殖酸物质、高分子芳香族物质和类腐殖酸物质;小球藻的添加增强了养猪沼液中DOMs的去除,同时降低了类腐殖酸的含量;(3)小球藻的添加明显增加了人工湿地中微生物的Alpha多样性。其中,门水平上,与反硝化作用相关的优势菌门(变形菌门)相对丰度明显增加;属水平上,亚硝化单胞菌属(Nitrosomonas)和湖沉积杆菌属(Limnobacter)在系统氨氧化和脱氮过程中发挥重要作用。该研究为小球藻作为外加碳源增强人工湿地脱氮除磷提供了理论支持。
Efficacy and Mechanism of Carbon Source Chlorella sorokiniana Enhanced Constructed Wetland for Treating Piggery Biogas Slurry
In response to the problems of insufficient denitrification and poor denitrification effect of traditional constructed wetlands for the treatment of livestock biogas slurry,in this study Chlorella sorokiniana was used as an additional carbon source for the construction of C.sorokiniana-constructed wetland systems to treat piggery biogas slurry.The effect of the system on the removal of water quality of piggery biogas slurry and the change rule of the dissolved organic matters(DOMs)through the addition of C.sorokiniana under different concentrations(0,0.25,0.5 and 1.0 g/L)were investigated,and the mechanism of en-hanced nitrogen removal was explored from the perspective of microorganisms.The results showed that compared with the constructed wetland without the addition of C.sorokiniana,the removal of total nitrogen was obviously increased up to 70.24%,and the removal of nitrate nitrogen reached up to 93.45%.Based on the three-dimensional fluorescence parallel factor analysis,the three components in DOMs were fulvic acid/humic acid-like substances,polymer aromatic substances and humic acid-like substances,while the addition of C.sorokiniana enhanced the removal of DOMs from piggery biogas slurry and reduced the content of humic acid-like substances.The addition of C.sorokiniana evidently increased the Alpha diversity of microorgan-isms in the constructed wetland.Particularly,at the phylum level the relative abundance of dominant bacteria related to deni-trification(Proteobacteria)increased obviously,while at the genus level the genera Nitrosomonas and Limnobacter played im-portant roles in the ammonia oxidation and denitrification processes of the system.This study provides theoretical support for the use of C.sorokiniana as an additional carbon source to enhance nitrogen and phosphorus removal in constructed wetlands.

constructed wetlandChlorella sorokinianapiggery biogas slurrynitrogen removal

滕玥、李天瑞、朱联东

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武汉大学资源与环境科学学院,湖北 武汉 430079

人工湿地 小球藻 养猪沼液 脱氮

国家自然科学基金项目湖北省杰出青年基金项目国家重点研发计划项目

222783232022CFA0702019YFD1101303

2024

环境科学与技术
湖北省环境科学研究院

环境科学与技术

CSTPCD北大核心
影响因子:0.943
ISSN:1003-6504
年,卷(期):2024.47(6)