Biological Characteristics of Enriched Culture of a Strain of Cryotolerant Autotrophic Ammonia-oxidizing Bacteria
An enrichment culture of Nitrosomonas was isolated from a municipal wastewater treatment plant.And effects of temperature,NH3-N concentration,and salinity on its ammonia oxidation activity and growth were studied,along with its effectiveness of low-temperature deammonification in treating black odor river-water,pulp/paper making wastewater and landfill leachate.The sole Nitrosomonas within this enrichment culture named Nitrosomonas mobilis NZ13 accounted for 99.85％of the total bacterial abundance and 16S rRNA and amoA gene sequences of NZ13 exhibited 97.40％and 99.79％similarity,respectively,to those of Nitrosomonas mobilis Nc2.Findings of the study presented the optimal growth temperature for this enrichment culture was 30 ℃,with a generation time being as low as 6.9 hours;the most suitable NH3-N concentration was 200～400 mg/L,the half inhibitory concentration was 840 mg/L,and the appropriate salinity for growth was 0.25％;and at a temperature as low as 7 ℃,the enrichment culture reached a maximum specific growth rate of 0.8 d-1,being capable of sig-nificantly upgrade the NH3-N removal rate in treating black odour river-water,pulp/paper making wastewater and landfill leachate at 13 ℃.Summarily,the enrichment culture can either be prepared rapidly at medium temperature or grows stably at low temperature,and moreover it could promote the removing of ammonia nitrogen from low-temperature effluents,thereby is demonstrating significant application potential.
万方数据
维普
