Isolation and Characterisation of Petroleum Hydrocarbon-degrading Bacteria and Laboratory Study on Simulation of Bioremediation
Efficiently oil-degrading bacteria screened from the soil around the refinery were used to explore the remediation ef-fect on the soil.A dominant surfactant-producing strain,numbered JZ3,was selected based on preliminary evaluation of emulsifi-cation index(E24)and oil degradation rate.The strain of JZ3 was identified as Bacillus paralicheniformis by 16S rDNA sequence analysis combing morphology,physiological and biochemical experiments.Two positive mutants Y1 and Y3 were obtained by three rounds of atmospheric and room temperature plasma.The degradation rates of the two mutants were increased to 41.78％and 45.10％,different from strain JZ3(P＜0.05),whose degradation of crude-oil was 39.77％within 7 d.Through the indoor simulation test of petroleum contaminated soil bioremediation,it is verified that the composite petroleum degrading bacteria with two kinds of bacteria Y1&Y3 have the best degradation effect on petroleum hydrocarbons,and the degradation rate can reach 65.32％in 30 d.This study would provide a theoretical basis for further remediation of petroleum contaminated soil.
万方数据
维普
