The Mechanism of Soil Microbial Community Structure Affected by Land Use Patterns in Guangze County
The changes of land use led by human activities greatly changed the physical and chemical properties of soil,and then affected the community structure and diversity of soil microorganisms.However,it is still unclear that how natural environmental factors,land use patterns and agricultural management measures affect soil microbial community structure and its driving factors.In order to clarify the response patterns of soil bacteria and nitrogen fixing microbial communities to land use in Guangze County of Wuyi Mountain,high-throughput sequencing technology was used to study the soil microbial community structure and diversity of five typical land use types(vegetable land(SC),paddy field(SD),Chinese fir forest(SM),bamboo forest(ZL),and primary secondary broad-leaved forest(KY)).The main influencing factors are also dis-cussed.The results showed that 16S rDNA and nifH microbial α diversity indices(Chao1 index and Shannon index)were significantly higher than SC index,followed by SM,KY and ZL,but Simpson index had the lowest value in SD soil.The dif-ferences of Chao1 index and Shannon index of 16S rDNA and nifH-containing microorganisms were mainly negatively corre-lated with slope in natural factors(P＜0.05),and positively correlated with soil silt content,moisture,pH,NH4+-N content and dissolved organic carbon(DOC)content(P＜0.05).The number of Firmicutes in SC and SD soil in agricultural land was 4.97 times and 9.30 times higher than that in other non-agricultural land(SM,KY,ZL),but the relative abundance of Rhizo-bium was significantly lower than that in other non-agricultural land types(84.88％).RDA analysis showed that the relative abundance of Firmicutes was mainly positively affected by soil NH4+-N content(P＜0.05),and the relative abundance of Rhi-zobium was mainly negatively affected by soil pH(P＜0.01).Comprehensive analysis showed that the bacterial community and Firmicutes were abundant in soil on agricultural land,but the nitrogen-fixing microorganism Rhizobium was rare,which was closely related to NH4+-N and DOC nutrient enrichment caused by fertilization.Therefore,appropriate supplemental fertil-izer measures could be taken in natural forest land to improve soil nutrients and promote microbial growth.
万方数据
维普
