首页|一株亚硝酸盐降解菌的分离鉴定与系统发育分析

一株亚硝酸盐降解菌的分离鉴定与系统发育分析

扫码查看
从水产养殖污泥中分离筛选了一株优良的亚硝酸盐降解菌AQ-3,其在1.0×107 cfu/mL时对50 mg/L亚硝酸盐的去除率高达99.47%.通过细菌自动鉴定系统(API ID32GN系统)以及16S rDNA系统发育分析,菌株AQ3被鉴定为鲍曼氏不动杆菌(Acinetobacter baumannii)(JF751054),其16S rDNA序列与基因库中不动杆菌属菌株的16S rDNA序列有99%的同源性,而且与鲍曼氏不动杆菌菌株14(FJ907197)的亲缘关系最近.此外,菌株AQ-3同时含有nirS基因、nirK基因和norB基因,这些基因对AQ-3高效降解亚硝酸盐具有重要的意义.
Isolation, identification and phylogenetic analysis of a nitrite degrading bacterium
A potential nitrite degrading bacterium AQ-3 was isolated and screened from the aquaculture sediment. The nitrite removal rate of AQ-3 was detected as 99. 47%. Strain AQ-3 was identified as Acinetobacter baumannii (JF751054) through API ID32GN bacterial identification system and 16S rDNA sequence analysis. Its 16S rDNA sequence had homology of 99% with those of Acinetobacter sp. submitted to GenBank.and showed the closest relative to Acinetobacter baumannii strain 14 (FJ907197). In addition,nirS gene、nirK gene and norB gene all existed in strain AQ-3. This study proved strain AQ-3 was a potential nitrite degrading bacterium,and enriched the microbial resources of nitrite degrading bacterium.

nitriteAcinetobacter baumanniiidentificationphylogenetic analysis

王会聪、曹海鹏、何珊、杨先乐

展开 >

上海海洋大学国家水生动物病原库,上海201306

上海师范大学教育系,上海200234

亚硝酸盐 鲍曼氏不动杆菌 鉴定 系统发育分析

国家“863计划”项目国家自然科学基金公益性行业(农业)科研专项现代农业产业技术体系建设专项

2011AA10A21631172430201203085CARS-46

2012

环境污染与防治
浙江省环境保护科学设计研究院

环境污染与防治

CSTPCDCSCD北大核心
影响因子:0.79
ISSN:1001-3865
年,卷(期):2012.34(4)
  • 8
  • 17