Study on the biological reaction mechanism of sulfate reduction and ammonia oxidation under organic condition
To investigate the biological reaction mechanism of sulfate reduction and ammonia oxidation(SRAO)under organic condition,the SRAO reaction system was established in an up-flow anaerobic sludge bed(UASB)reactor,where ammonia nitrogen and sulfate could be removed simultaneously.The SRAO process in the UASB was found to be carried out in steps and layers after mass balance calculation,activity measurement and microbial analysis.First,in the middle and lower sludge,ammonia nitrogen reacted with sulfate under the action of microorganisms to produce sulfur ion and nitrate nitrogen.Subsequently,in the upper and middle sludge,sulfur ion reacted with nitrate nitrogen by sulfur-dependent autotrophic denitrification microbes to form sulfate and nitrogen.Since the accumulation of sulfur ion in the reactor inhibited SRAO microbial activity,the second reaction step was the rate-limiting step of the overall reaction.To solve the inhibition of sulfur ion to the SRAO microbes is a big challenge in future for the development of SRAO.
up-flow anaerobic sludge bedsulfate reduction and ammonia oxidationsulfur-dependent autotrophic denitrificationmicrobial community analysis