Variation of intracellular and extracellular ARGs abundance in waste activated sludge during thermal-alkali dewatering
Waste activated sludge(WAS)contains high abundance of antibiotic resistance genes(ARGs).With the WAS dewatering,intracellular ARGs(iARGs)may gradually transform into extracellular ARGs(eARGs).In order to explore the characteristics of ARGs abundance in WAS dewatering,the change of iARGs and eARGs abundance was analyzed in the WAS before and after thermal-alkali pretreatment(TAP).The results showed that TAP could effectively enhance the dewatering effeciency of WAS,and the optimal condition was determined by response surface methodology(RSM).The dewatering effeciency reached 41.0%after 80 min at pH=11 and 90℃.iARGs were transformed into eARGs during WAS dewatering.The common logarithm of absolute abundance of eARGs increased by 0.2-1.8 significantly after TAP,which increased the risk of ARGs spread and brought great challenges to WAS dewatering.