The composite biological effects on Carassius auratus of different kinds of microplastics and nano-ZnO
To explore the influence on the compound effects of microplastics(MPs)and other granular pollutants caused by different types of MPs,1 mg/L MPs in the same particle size(500 nm)were selected as the target pollutants,including polystyrene microplastics(PS MPs),polyvinyl-chloride microplastics(PVC MPs)and polymethyl methacrylate microplastics(PMMA MPs),together with 1 mg/L nano-ZnO.Carassius auratus was selected as the test creature.In order to explore the status and toxicity effects of MPs and nano-ZnO composition,the material characterization,the water matching particle size,the Zeta potential,the concentration of Zn2+,the accumulation of Zn in the fish and the oxidative damage of the fish liver were monitored.It was showed that the surface elements of MPs were different,mainly being C and O.PMMA MPs had larger water size and more unstable system,single or compound.Nano-ZnO had lower absolute value of Zeta potential,more likely to be reunited,and composition increased the absolute value of Zeta potential,enhanced the stability.MPs compound forced nano-ZnO to release Zn2+,particularly PS MPs increasing the content of Zn in water and eyes,gill and meat of the fish.The composite exposure showed greater oxidative damage.PS MPs had high molecular polarity,strong surface electrostatic action and poor system stability,which combined with nano-ZnO promoted the release of Zn2+and enhanced the compound toxicity effect.