Study on the environmental estrogens degradation function of phage in activated sludge
As environmental estrogens(EEs)are discharged into natural water bodies along with wastewater,it will cause great risks to the ecological environment and human health.Using metagenomic sequencing technology to study ordinary activated sludge,as well as bisphenol A(BP A),dibutyl phthalate(DBP)and nonylphenol(NP)enriched sludges,it was found that the phages in ordinary activated sludge and EEs enriched sludges were mainly Siphoviridae,Myoviridae and Podoviridae detected.However,there were significant differences in the relative abundance composition of phages in different sludges.Through functional gene annotation of phage genomes,it was found that the phage genomes of Siphoviridae in BP A-and NP-enriched sludges encoded phenol monooxygenase and dehydrogenase,respectively.Monooxygenase and dehydrogenase would contribute to remove aromatic compounds by mineralization.By analyzing parasitic relationships between phages and bacteria,it was found that phages were widely parasitic in various bacteria.But it was Siphoviridae in BPA-enriched that had great potential auxiliary metabolic function for EEs,which encoded monooxygenase gene with BPA metabolic function.
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