The study aimed to investigate the removal efficiency of antibiotics from the simulated swine wastewater by four microalgae treatment systems enhanced with gibberellin(GAs):Chlorella vulgaris monoculture,Chlorella vulgaris+Clonostachys rosea,Chlorella vulgaris+Ganoderma lucidum,Chlorella vulgaris+Pleurotus pulmonarius.Different concentrations of GAs were added to analyze the daily production,specific growth rate,and chlorophyll a content changes in microalgae within these systems,as well as to monitor the removal rates of tetracycline(TC),oxytetracycline(OTC),sulfamethazine(SMZ),sulfamethoxazole(SMX),and sulfamethizole(SMM)from the simulated swine wastewater.The optimal GAs concentration and superior microalgae treatment technology were identified.The results indicated:1)the best treatment effect was achieved when the initial mass concentration of GAs was 50 mg/L for all four microalgae treatment systems.2)The treatment system of Chlorella vulgaris+Clonostachys rosea outperformed the other three treatment systems,with average antibiotic removal rate reaching 94.41%±2.79%(TC),90.31%±5.24%(OTC),59.15%±5.48%(SMZ),61.54%±5.82%(SMX),and 54.97%±4.38%(SMM)on the 7th day.This treatment technology was more effective in reducing tetracycline-class antibiotic than sulfonamide-class antibiotic.3)In terms of purification time,the best effect was observed on the 7th day.The findings could provide a reference for the study and application of algae-fungus treatment technology for the removal of antibiotic.