Research on the resource utilization of kitchen waste based on microbial fermentation
To promote the harmless and resourceful utilization of kitchen waste,high enzyme-producing bacteria were screened and isolated.The growth and enzyme production patterns of these strains were analyzed,and composite engineered bacteria were subsequently constructed for the fermentation treatment of kitchen waste.The results showed that among the five isolated strains,Lichthemia corymbifera produced amylase and cellulase with maximum activities of 151 U/g and 135.5 U/g,respectively,while its biomass increased steadily within 156 h.Rhizopus oryzae exhibited a biphasic growth pattern,with protease activity reaching 1 260 U/g after 156 h of fermentation.Pichia pastoris achieved maximum lipase activity(8.40 U/g)at 108 h,and Bacillus subtilis exhibited the highest protease activity(700 U/g)at 156 h.Bacillus licheniformis proliferated rapidly and produced amylase,protease,cellulase,and lipase during cultivation.When the five strains were mixed in a specific ratio and used for the fermentation of kitchen waste,the biomass nucleic acid content was found to peak on the 4th day,and its growth was observed to show similar trends with amylase activity,protease activity,cellulase activity,and lipase activity.During 5-10 d of fermentation,the temperature rose above 50 ℃ due to active metabolism,then decreased after 11d.The pH fluctuated during the early fermentation stage but stabilized around 7 after 11 d.The seed germination index increased from 10.0％before fermentation to over 80％after fermentation.After fermentation treatment,the effective viable count,organic matter,and seed germination index of the kitchen waste were found to meet the requirements of"Microbial organic fertilizers"(NY 884-2012).
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