Optimization of degradation of expanded corn straw by cellulase and hemicellulase
Cellulose and hemicellulase were used to degrade the corn straw pretreated by expanded in order to improve the sugar yield which compared with degraded only by cellulose.On basis of single factor experiment,and use reducing sugar yield as response value,optimization experiment was designed by response surface.By analysis of testing data,results showed that the best process for the expanded corn straw enzymatic hydrolysis was pH of 4.8,liquid to solid ratio of 13 ∶ 1,hydrolysis time of 60 h,enzyme concentration of 6 g/L,temperature of 51℃.Compared with the results which degraded only by cellulase,the reducing sugar yield increased 14.3％ and reached to 24％.The study of synergetic enzymatic hydrolysis provides a new way to degrade the lignocellulose material.
国家科技期刊平台
NSTL
万方数据
维普
