Microbial community structure of anoxic-oxic-settling-anaerobic sludge reduction process characterized by phospholipid fatty acids analysis
With the traditional anoxic-oxic process as a reference,the variation of microbial biomass and community structure in the anoxic-oxic-settling-anaerobic (A + OSA) process was studied by the technology of phospholipid fatty acid (PLFA) analysis.The results showed that the composition of PLFAs in the sludge reduction process and reference process were mainly saturated fatty acid,branched fatty acid and monounsaturated fatty acid,the contents of cyclopropane fatty acid and OH-branched fatty acid were relatively small.Sludge decay in the sludge holding tank (SHT) was main reason for the sludge reduction in the A + OSA process,gram-negative (G-) bacteria and aerobic microorganisms were the mainly microorganisms contributed to the sludge reduction,and the sludge reduction process could inhibit fungal growth according to the variation of content.Due to the insertion of SHT,the microbial community structures of the sludge reduction process was different from reference process,and the similarity of microbial community structures between anoxic tank and oxic tank in the sludge reduction process was improved.
phospholipid fatty acidoxic-settling-anaerobic (osa) processsludge reductionmicrobial community structure