Start-up of SAD process treating phenol-containing wastewater and its sludge characterizations
The anaerobic ammonium oxidation(Anammox)granular sludge was used as the basis to start the simultaneous anammox and denitrification(SAD)process in an upflow anaerobic sludge bed(UASB)reactor by adding sodium acetate and then replacing it with phenol step by step.The denitrification and carbon removal performance of the coupled system as well as the characteristics of the granular sludge was investigated during the startup process.The results showed that when the system was successfully started,the removal rates of TN,COD,phenol were 84.78％,85.23％,84.09％,respectively,the nitrogen removal rate(NRR)and carbon removal rate(CRR)were 0.73,0.31 kg·(m3·d)-1,respectively.Phenol had a stronger inhibitory effect on the Anammox bacteria,the denitrifying bacteria proliferated on the surface of the granular sludge of Anammox.The content of soluble microbial products(SMP)and extracellular polymeric substances(EPS)increased.FT-IR and 3D-EEM analyses further confirmed that protein(PN)was the main components of SMP and EPS,tryptophan-like and tyrosine-like analogs played an important role in resisting phenol toxicity and maintaining the stability of granular sludge.
anammoxdenitrificationphenol-containing wastewaternitrogen and carbon removalsgranular sludge
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