Synergistic effects of different habitats ammonia concentration and suspended sludge on anammox biofilm characteristics
Biofilm technology is the key to the application of anaerobic ammonia oxidation process,but there are few reports on the characteristics of biofilms formed under synergistic effect of different ammonia nitrogen concentrations and suspended sludge.In this study,in a plug-flow fixed biofilm-activated sludge reactor,the biofilm sludge concentration and thickness under high ammonia nitrogen concentration conditions were greater than those under low ammonia nitrogen concentration,but the latter had higher anammox bacteria abundance((4.91±0.65)×109 copies·g-1,P＜0.05)and activity(6.53 mg·(g·h)-1).High-throughput analysis showed that Candidatus Brocadia was the major anaerobic ammonia oxidation bacteria in both biofilms and suspended sludge,and there was no significant difference in abundance between the two types of biofilms.The relative abundance of Candidatus Jettenia was significantly higher in biofilms at low ammonia nitrogen concentrations than at high ammonia nitrogen concentrations,but the abundance of Candidatus Kuenenia was the opposite.Comprehensive analysis revealed that the attachment growth of anaerobic ammonia oxidation bacteria was related to the initial colonization of suspended sludge community diversity,while the distribution of low abundance AnAOB was influenced by different microhabitats.This study suggests that the synergistic effect of different ammonia nitrogen concentrations and suspended sludge types on biofilms should not be neglected.
partial nitrification-anammoxbiofilmammonia nitrogen concentrationsuspended sludgemicrobial community
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