Effects of different size Aspergillus-aerobic granular sludge microenvironment on nitrogen and phosphorus removal capacity
Aiming at the problems of slow granulation and poor stability of aerobic granular sludge in laboratory.Aspergillus,obtained by screening and separation in the laboratory,which can form into balls automatically in the process of oscillation culture,was added to the flocculent sludge system in reverse as a carrier to promote the granulation process.The performance of the large(3.0 mm＜D ≤ 5.0 mm),medium(1.5 mm＜D ≤3.0 mm),and small(D ≤1.5 mm)AGS were compared,and when combined with the monitoring experimental data of pollutant removal performance,and the evolution of bacterial community structure were analyzed.The results showed that the large size was the most optimal AGS size compared to the small and middle,which could promote the secretion of extracellular polymeric substances,thus improving the granulation performance,maintaining the stable structure of particles,and showing good comprehensive ability in the treatment of pollutants.In the reactors with different particle sizes,microbial community analysis showed that the fungus Aspergillus,as the dominant carrier of aerobic granular sludge,accounted for 4.17％,85.67％,47.20％,and 58.00％in the flocculent sludge,large,middle and small size reactors.In addition,the proportion of bacteria NOB and PAO in the large size adding reactor was significantly higher than those in the other two reactors.Denitrification bacteria such as Ferruginibacter and Castellaniella had high abundance and had obvious advantages in nitrogen and phosphorus removal.The removal rate of ammonia nitrogen,total nitrogen,total phosphorus and nitrate in L-AGS system within 6 h was 77.2％,52.8％,49.2％and 93.9％,respectively.
aerobic granular sludgeAspergillusparticle sizemicroenvironmentnitrogen and phosphorus removal
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