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富铁环境中甲烷氧化菌群落结构与甲烷氧化特性

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近年来湿地潜在甲烷排放备受关注,牟尼沟草海富铁湿地具有十分重要的生态地位,而目前有关该区域甲烷氧化菌群落结构与氧化特性的研究相对较少.通过第二代高通量测序技术探究了草海富铁湿地中甲烷氧化菌的群落结构及功能并从中筛选出一株优势甲烷氧化菌在富铁溶液中进行实验探究.测序结果表明,优势甲烷氧化菌为Type Ⅰ型的甲基杆菌(Methylobacter)和甲基单胞菌(Methylomonas),RDA分析结果表明,pH和ORP是影响甲烷氧化菌群落的关键环境驱动因素.结果表明,反应过程中菌株会分泌腐殖质等产物将胞内电子转移到胞外,使溶液中的Fe(Ⅲ)作为电子受体.并且在富铁溶液中甲烷氧化效率提高了 10.8%左右,7 d内菌株能够完全氧化瓶内的甲烷气体.以上结果以期为湿地甲烷减排提供理论参考依据.
Community structure and methane oxidation characteristics of methanotrophs in iron-rich environment
In recent years,the potential methane emission from wetlands has attracted much attention.The iron-rich wetland in Caohai of Munigou has a very important ecological status.However,there are relatively few studies on the community structure and oxidation properties of methanotrophs in this area.The next generation sequencing technology was used to study the community structure and function of methanotrophs in the iron-rich wetland of Caohai,and a type of dominant methanotrophs was screened out to conduct experiment tests in the iron-rich solution.Sequencing results showed that the dominant methanotrophs were Type Ⅰ Methylobacter and Methylomonas,and RDA analysis indicated that pH and ORP were the key environmental drivers affecting the methanotrophs community.The experimental results showed that during the reaction,the strain could secrete products like humus to transfer intracellular electrons to extracellular ones.Therefore,Fe(Ⅲ)in the solution could act as an electron acceptor.The oxidation efficiency of methane in the iron-rich solution increased by about 10.8%,and the strain could completely oxidize the methane gas in the flask within 7 days.These results are expected to provide a theoretical reference for methane emission reduction in wetlands.

iron-rich wetlandmethanotrophscommunity structuremethane oxidation

杨慧娴、代群威、杜伟旗、山静、曾翔萍

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西南科技大学环境与资源学院绵阳 621010

富铁湿地 甲烷氧化菌 群落结构 甲烷氧化

国家自然科学基金资助项目区域创新发展联合基金重点项目

41877288U21A2016

2024

环境工程学报
中国科学院生态环境研究中心

环境工程学报

CSTPCD北大核心
影响因子:0.804
ISSN:1673-9108
年,卷(期):2024.18(2)
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