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梯度曝气A2O2-MBR工艺强化同步硝化反硝化效能

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针对城镇污水厂对低C/N比污水脱氮效果差、能耗高的问题,研发了梯度曝气A2O2-MBR工艺,设置单独的低氧池(O,池)和好氧池(O2池),O1和O2池DO质量浓度逐步降至0。6 mg·L-1和1。7 mg·L-1。进水碳氮比(C/N)从6。0逐步降至5。0和4。0,总氮(TN)去除率保持在85%左右。O1和O2池同步硝化反硝化效率保持在90%左右,O池(O1池+O2池)和膜池对TN去除的贡献在82。5%以上,对COD去除的贡献在64。9%~83。2%。高通量测序结果表明,在低DO低C/N比条件下,系统中亚硝酸盐氧化菌(nitrite-oxidizing bacteria,NOB)丰度下降,反硝化聚糖菌(denitrifying glycogen accumulating organisms,DGAOs)丰度增加;反硝化菌类群丰富,其中Pseudomonas的丰度最高,Thauera和Zoogloea在低C/N比条件下丰度增加。大部分TN通过常规异养反硝化菌(denitrifying bacteria,DNB)去除,DGAOs的贡献较小。与启动阶段相比,O1池和O2池总曝气量下降了 44。2%。结果表明,通过采用梯度曝气方式,并逐步降低DO浓度,可促进功能菌的富集,强化SND作用,改善TN去除效果,并降低能耗。
Enhanced SND performance of step-wise aeration A2O2-MBR process
Aiming at poor denitrification effect and high energy consumption when treating the wastewater with the low C/N ratio in wastewater treatment plant(WWTPs),a step-wise aeration A2O2-MBR process was developed.The separated low-oxic zone(O1)and oxic zone(O2)were design with the DO concentrations decreased gradually to 0.6 mg·L-1 and 1.7 mg·L-1,respectively.When the influent C/N ratio decreased from 6.0 to 5.0 and 4.0,the total nitrogen(TN)removal efficiency maintained at around 85%.The simultaneous nitrification and denitrification(SND)efficiency of the O1 and O2 zones kept at around 90%.Both O zones(O1+O2)and membrane zones contributed to above 82.5%of TN removal,and 64.9%~83.2%of COD removal.High-throughput gene sequencing showed that,under low DO and low C/N ratio conditions,the abundance of nitrite-oxidizing bacteria(NOB)decreased,while the abundance of denitrifying glycogen accumulating organisms(DGAOs)increased.Rich denitrifiers occurrred,of which the abundance of Pseudomonas was highest.Besides,the abundance of Thauera and Zoogloea increased under low C/N ratio condition.Most of TN was removed by ordinary denitrifying bacteria(DNB),rather than DGAOs.Compared with the startup stage,the total aeration rate of the O1 and O2 zones decreased by 44.2%.The results showed that the step-wise aeration mode could gradually lower the DO concentrations,enrich the functional bacteria,then enhance the SND effect,increase TN removal,save the energy consumption.

municipal wastewaterbiological nitrogen removalsimultaneous nitrification and denitrification(SND)DO controlhigh-throughput gene sequencing

刘钢、王丽、温榛煌、谌建宇、黎京士、何伟、梅嘉鑫、张宇

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生态环境部华南环境科学研究所,广州 510655

中国建筑第四工程局有限公司,广州 510000

广东环境保护工程职业学院,佛山 528216

城镇污水 生物脱氮 同步硝化反硝化 溶解氧控制 高通量测序

中央级公益性科研院所基本科研业务费专项中建四局科技研发课题

PM-zx703-202104-130CSCEC4B-2021-KTA-6

2024

环境工程学报
中国科学院生态环境研究中心

环境工程学报

CSTPCD北大核心
影响因子:0.804
ISSN:1673-9108
年,卷(期):2024.18(4)
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