Enhanced SND performance of step-wise aeration A2O2-MBR process
Aiming at poor denitrification effect and high energy consumption when treating the wastewater with the low C/N ratio in wastewater treatment plant(WWTPs),a step-wise aeration A2O2-MBR process was developed.The separated low-oxic zone(O1)and oxic zone(O2)were design with the DO concentrations decreased gradually to 0.6 mg·L-1 and 1.7 mg·L-1,respectively.When the influent C/N ratio decreased from 6.0 to 5.0 and 4.0,the total nitrogen(TN)removal efficiency maintained at around 85%.The simultaneous nitrification and denitrification(SND)efficiency of the O1 and O2 zones kept at around 90%.Both O zones(O1+O2)and membrane zones contributed to above 82.5%of TN removal,and 64.9%~83.2%of COD removal.High-throughput gene sequencing showed that,under low DO and low C/N ratio conditions,the abundance of nitrite-oxidizing bacteria(NOB)decreased,while the abundance of denitrifying glycogen accumulating organisms(DGAOs)increased.Rich denitrifiers occurrred,of which the abundance of Pseudomonas was highest.Besides,the abundance of Thauera and Zoogloea increased under low C/N ratio condition.Most of TN was removed by ordinary denitrifying bacteria(DNB),rather than DGAOs.Compared with the startup stage,the total aeration rate of the O1 and O2 zones decreased by 44.2%.The results showed that the step-wise aeration mode could gradually lower the DO concentrations,enrich the functional bacteria,then enhance the SND effect,increase TN removal,save the energy consumption.
municipal wastewaterbiological nitrogen removalsimultaneous nitrification and denitrification(SND)DO controlhigh-throughput gene sequencing