Influence and mechanism of drinking water disinfection strategies on antibiotic resistance risk
To explore the impact and mechanism of drinking water disinfection on the risk of antibiotic resistance,the operation of different disinfection processes was simulated under pilot conditions in this study,and the metagenomic approach based on high-throughput sequencing was employed to analyze the effects of different disinfection processes on the abundance,potential mobility,and potential pathogenicity of antibiotic resistance genes(ARGs).Their control effectiveness on ARGs risk was comprehensively evaluated and the underlying mechanisms were deciphered.The results indicated that ozone-chlorine disinfection(O-C)significantly reduced the total relative abundance of ARGs,while antimicrobial resin-chlorine disinfection(AR-C)and antimicrobial resin-ultraviolet disinfection(AR-UV)significantly increased the total relative abundance of ARGs.Simultaneously,AR-C and AR-UV increased the total relative abundance of mobile genetic elements(MGEs),promoting the co-occurrence of ARGs and MGEs.In addition,these two disinfection processes increased the total relative abundance of virulence factor genes(VFGs),of which the offensive and other VFGs made the most contribution.AR-UV also facilitated the co-occurrence of ARGs and VFGs,and the gene combination of multidrug resistance genes and VFGs was the main co-occurrence mode.Overall,AR-C and AR-UV increased the potential risk of ARGs.Disinfection altered the composition and abundance of ARGs potential hosts,and host changes and horizontal gene transfer were the key factors for the variation of ARGs during the disinfection process.
drinking water disinfectionantibiotic resistance genesrisk of antibiotic resistancemetagenomics
国家科技期刊平台
NSTL
万方数据
