Effects of nitrogen sources addition on bacterial communities and their degradation of petroleum hydrocarbons in oil-based drilling cuttings
To investigate the impact of different types of nitrogen sources addition on bacterial communities and their degradation of petroleum hydrocarbons in oil-based drilling cuttings.The differences in bacterial community diversity,composition,and functions during the biological treatment of oil-based drilling cuttings with six different nitrogen sources were studied,and the correlation between bacterial community changes and petroleum hydrocarbon degradation was analyzed by high-throughput sequencing technology and conventional methods for determining petroleum hydrocarbon.The results showed that nitrogen sources addition caused significant differences in the bacterial community structure in oil-based drilling cuttings,but Acinetobacter and Rhodococcus were still the dominant genera in each sample,and nitrogen sources addition reduced the diversity and richness of the bacterial community in oil-based drilling cuttings;The main differences(P ≤ 0.001)of bacterial communities in oil-based drilling cuttings were Novosphingobium,Caulobacter,Bacillus and Methylobacterium among the six nitrogen sources addition;The addition of(NH4)2HPO4 and yeast extract effectively promoted the degradation of the main petroleum hydrocarbons in oil-based drilling cuttings,with most degradation rates reaching over 60％;The main bacteria related to the degradation of major petroleum hydrocarbon components in oil-based drilling cuttings were Acinetobacter,Rhodococcus,Bacillus,Stenotrophomonas,Sphingomonas and Brevundimonas.It was found that nitrogen sources addition could be beneficial for the degradation of the main components of petroleum hydrocarbons in oil-based drilling cuttings,which could provide a research basis for the on-site application of oil-based drilling cuttings biodegradation technology.
万方数据
维普
