Mycelial pellets enhance the construction,performance and microbial community characteristics of salt-tolerant aerobic granular sludge
based on the Martelella sp.AD-3 moderately halophilic bacteria coupled with activated sludge,an enhanced degradation system for salt-tolerant organic pollutants,the impact of exogenous mycelium pellets on the construction of a SAGS system and the removal of PAHs pollutants was explored.A salt-tolerant fungus,Penicillium sp.L22,was obtained by screening the intertidal sludge of Hangzhou Bay as the inoculation source under the conditions of 5％salinity and glucose and phenanthrene as carbon sources.It was found that under the conditions of pH 7 and salinity 3％,the growth and degradation performance of mycelial pellets reached the best.The above-mentioned mycelial pellets were added to the Martelella sp.AD-3 bacteria and activated sludge enhancement system.Further research found that compared with the control group(AD-3 bacteria+activated sludge),the experimental group(mycelial pellets+AD-3 bacteria+activated sludge)although the granulation was slow and the formed particles were small(the average particle size was reduced by 19.3％),the formed particle structure was more compact and stable,and the biomass of the reactor was large(VSS increased by 3.2 times),and the sewage Mud settlement was better(24.4％reduction in SVI5).Through the study of extracellular polymers(EPS),it was found that the addition of mycelial pellets increased the content of extracellular protein in the sludge in the reaction system,thereby promoting sludge agglomeration.In terms of the degradation of organic pollutants,compared with the control group,the removal rate of COD in the third stage of the mycelial pellets experimental group increased by 20.5％,and the specific degradation activity of the representative model pollutant phenanthrene increased by 53.1％.Using high-throughput gene sequencing technology analysis,it was found that the relative abundance of Martelella was significantly positively correlated with the specific degradation activity of sludge,indicating that the addition of mycelial pellets may enhance the persistence of AD-3 bacteria(Martelella)and thus strengthen the phenanthrene.degradation.In addition,the degradation of mycelium Penicillium and phenanthrene also had a significant positive correlation.This study reveals the community succession characteristics of mycelial pellets enhanced SAGS system,and provides technical support and theoretical basis for the enhanced treatment of high-salt organic wastewater.
万方数据
维普
