Co-metabolic characteristics of phenanthrene by a strain of Ochrobactrum sp.FP1 under the addition of substrates
Polycyclic aromatic hydrocarbons(PAHs)have low degradability and accumulate in natural environment for a long time,causing environmental pollution and biological toxicity.Ochrobactrum sp.FP1 is an efficient petroleum degrading bacterium isolated from oil contaminated soil.The growth characteristics and optimum growth conditions of Ochrobactrum sp.FP1 were studied by coated plate counting and High Performance Liquid Chromatography(HPLC).The growth characteristics and phenanthrene degradation ability of bacteria in the presence of different external substrates such as glucose,glycerol,catechol,salicylic acid and rhamnoolipid were studied.The results showed that Ochrobactrum sp.FP1 could grow normally at pH 5～8 and 20～40 ℃ with phenanthrene content of 50·mg L-1,and the optimum growth temperature was 30 ℃ and pH 7.The addition of substrates glucose,glycerol and rhamnose-lipids had a good promotion effect on the growth of the strain,and glycerol was the most obvious promotion effect,while salicylic acid and catechol had an inhibitory effect on the growth of the strain.The results of HPLC analysis showed that compared with the control group,the degradation rates of the additional substrates glycerol,glucose and rhamnose-grease treatment groups were increased by 21.58％,15.38％and 6.76％,respectively,while the degradation rates of salicylic acid and catechol treatment groups were decreased by 46.27％and 70.31％,respectively.The change of bacterial degradation rate of phenanthocyanin was positively correlated with its growth ability.High glucose concentrations inhibited the metabolism of phenanthrene by the strain,and the degradation enzymes of phenanthrene in Ochrobactrum sp.FP 1 were inducible enzymes.The results of this study can provide theoretical reference for bacterial co-metabolism of polycyclic aromatic hydrocarbons.
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