Treatment effect of digested piggery wastewater by biological aerated filter with different fillers and microbial community response
In this study,three groups of three-stage and tandem biological aerated filters(BAFs)were constructed with ceramic,zeolites and gravels as fillers,respectively.Under the conditions with different C/N ratio in BAF influent,the treatment effects and differences of COD、NH4+-N、TN、TP and antibiotic in digested piggery wastewater(DPW)were investigated.The 16s rRNA high-throughput sequencing was used to analyze the biofilms of BAFs.The results showed that the BAF with ceramic as filler had the best effect on removing pollutants,and the average removal rates of COD,NH4+-N,TN,and TP in DPW were 93.77%~94.14%,99.44%~99.89%,64.63%~85.23%,and 92.68%~95.74%,respectively;and the average removal rates of SMZ2,OTC,TC,CFX,and OFX ranged from 94.72%to 97.07%,94.85%to 95.27%,83.17%to 92.05%,47.62%to 65.02%,and 52.76%to 69.45%,respectively.After the C/N ratio in BAF influent increased,the treatment efficiency of TN by the three groups of BAFs increased significantly(P<0.01),and the removal rates of SMZ2,TC and CFX also increased(P<0.05),while the removal rates of NH4+-N and TP increased insignificantly.According to the 16S rRNA sequence analysis,the relative abundance of Proteobacteria and Bacteroidetes was high in the bacteria on the biofilm of the three groups of BAF;Thauera,Zoogloea,Flavobacterium and Arcobacter were the dominant bacteria in the bacteria on the biofilm of the three groups of BAF,of which the genus of Thauera,being mainly the denitrifying bacteria,had the highest relative abundance of 36.27%in the ceramic group,leading to its good removal efficiency of pollutant.This study can provide a reference for the treatment of aquaculture wastewater using biofilter technology.