Influence of protein degradation regulation on the mesophilic phase of aerobic fermentation of sludge
Based on analyzing characteristics of protein decomposition during the mesophilic phase,the study proposed strategies for regulating protein degradation and further investigated the impact of these regulatory measures on humification and the structure of microbial communities.After conducting batch experiments using sewage sludge,sawdust,and mature compost as raw materials,it was found that the changes in concentration of degradable proteins during the mesophilic phase could be analyzed by the initial protein concentration and the duration of mesophilic phase.After analyzing the content of degradable proteins and temperature changes during the mesophilic phase using the first-order kinetic equation and the heat balance equation,it was found that both the rate of temperature increase and the residual protein rate rose with the increase in initial protein content.After increasing the proportion of sludge mass from 62%to 68%,the duration of thermophilic phase for the treatment group was extended from 6 days to 10 days.Compared to the control group,the treatment group showed an increase in effective accumulated temperature by 14.39%,humic acid content by 26.59%,and the degree of humification by 38.57%.Bacillus was the dominant genus during the thermophilic phase,Niabella and Ureibacillu was the relatively dominant genus during the maturation phase.Increasing the proportion of sludge was beneficial for the growth of thermophilic bacterial communities such as Bacillus,promoting the ability to consumption of organic matter,and thereby promoting the humification process during aerobic fermentation of sludge.