The influence of membrane filtration and post installation process of terminal water purifiers on the structural characteristics of microbial communities in water
To investigate the microbial contamination of terminal water purifiers which is referred to as water purifiers and the differences in community structure,this study utilized water purifiers to treat water sourced from the urban pipeline network over a period of 150 days.The nanofiltration effluent was connected to either a pressure storage device or a post activated carbon filter for treatment.The influent to the water purifiers,along with three groups of effluent—nanofiltration effluent alone,nanofiltration combined with a pressure bucket,and nanofiltration combined with activated carbon—was analyzed using 16S rRNA amplicon technology.The results showed that three treatment processes,namely nanofiltration,nanofiltration/pressure bucket,and nanofiltration/activated carbon,resulted in differences in water quality indicators,alpha diversity,and microbial community structure in the effluent of the water purifier.The dominant bacterial phyla in the effluent of the three groups of water purifiers are Proteobacteria,Actinobacteria,and Bacteroides.However,after 90 days of operation,higher levels of Firmicutes and Bacteroides were detected in the effluent of nanofiltration/pressure bucket and nanofiltration/activated carbon compared to the effluent of nanofiltration alone,indicating that nanofiltration/pressure bucket and nanofiltration/activated carbon can significantly enrich microorganisms.A total of 14 risk bacterial genera were detected in the effluent of the three groups of water purifiers,with Neisseria and Rothia having the highest relative abundance.The number of functional expression genes in the effluent of the water purifier is higher than that in the influent,with the highest number of functional expression genes in the effluent of nanofiltration/activated carbon.
terminal water purifiersnanofiltrationnanofiltration combined with a pressure bucketnanofiltration combined with activated carbon16S rRNA amplicon technology
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