The impact of different types of organic carbon sources on sulfur autotrophic partial denitrification processes
The study investigated sulfur autotrophic partial denitrification(SAPD)process by utilizing an anaerobic sequencing batch reactor(ASBR),with thiosulfate serving as the electron donor.The effects of different organic carbon sources(methanol,ethanol,sodium acetate,glucose)on SAPD nitrite accumulation were investigated by batch experiments,and then the results of batch experiments were applied to ASBR system to verify the enhancement effect.When the S/N ratio was 1,the ASBR system successfully operated,achieving a nitrate removal efficiency(NRE)of 95.89％and a nitrite accumulation efficiency(NAE)of 52.40％.Results from batch experiments with a C/N ratio of 0.5 indicated that the effectiveness of nitrite accumulation was as follows:sodium acetate＞glucose＞methanol～ethanol＞no carbon source.Among these,sodium acetate had the most significant enhancement on nitrite accumulation,increasing the NAE from 50.52％to 61.09％,while the elemental sulfur production rate decreased from 40.43％to 30.21％.The optimization results in ASBR show that,the addition of sodium acetate further enhanced the NO3--N removal and NO2--N accumulation ability.The NAE increased from 65.00％to 73.70％,and the NRE also improved from 80.6％to 89.15％.High-throughput sequencing results indicated that the addition of sodium acetate significantly increased the abundance of Proteobacteria in the SAPD system,while the activity of Firmicutes was inhibited.The relative abundance of Thiobacillus increased from 6.83％to 10.32％,and the relative abundance of Terrimonas decreased from 10.68％to 1.20％.Overall,the addition of low concentrations of organic matter can enhance the overall effectiveness of the SAPD process.
sulfur autotrophic partial denitrificationnitrate removalorganic carbon sourceNO2--N accumulationmicrobial community
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