Association of arsenic exposure with DNA damage of six mitochondrial genes in arsenic factory workers
Objective To explore the relationship between DNA damage of six mitochondrial genesand urinary arsenic metabolism in workers exposed to arsenic.Methods The content of arsenic in the urine was determined by hydride generation-atomic absorption spectrophotometry,and the DNA damage levels of six mitochondrial genes(ND1,ATP6,ATP8,COX 1,COX2,COX3)in the peripheral blood were detected by real-time fluorescence quantitative PCR among occupational arsenic exposure workers in an arsenic factory in Yunnan(the exposure group,n=78)and population without history of arsenic exposure(the control group,n=24)from 2017 to 2018.Results The contents of inorganic arsenic,monomethyl arsenic,dimethylated arsenic,and total arsenic in the urine of the arsenic-exposed group were significantly higher than those in the control group(P＜0.05).The percentage of dimethylated arsenic,dimethylated arsenic was significantly higher than that in the control group(P＜0.05),while the dimethylation index was significantly lower than that in the control group(P＜0.05).The DNA damage levels of the six mitochondrial genes in the occupational arsenic exposure group were significantly higher than those in thecontrol group(P＜0.05).The damage levels of the six mitochondrial genes were positively correlated with inorganic arsenic,monomethyl arsenic,dimethylated arsenic,and the percentage of monomethyl arsenic in urine and negatively correlated with the percentage of dimethylated arsenic(P＜0.05).The DNA damage levels of six mitochondrial genes in high SMI group were significantly lower than those in low SMI group(P＜0.05).Conclusion Occupational arsenic exposure induces mitochondrial DNA damage,which is related to the methylation metabolism of arsenic in the body.
万方数据
维普
