Antagonistic effect of ginsenoside Rh2 on lung injury in mice with cigarette smoke-induced chronic obstructive pulmonary disease
Objective Exploring the antagonistic effect of ginsenoside Rh2 on lung injury in BALB/c mice with chronic obstructive pulmonary disease(COPD)induced by cigarette smoke.Methods Fifty healthy 6-8-week-old SPF grade BALB/c female mice were randomly divided into a blank control group,a COPD model group,a Pumicor Lingshu group,and a group treated with 0.5 and 1 mg/ml ginsenoside Rh2,with 10 mice in each group.The COPD model group,Pumicoline Shu group,0.5 mg/ml ginsenoside Rh2 group,and 1 mg/ml ginsenoside Rh2 group were all passively smoked once a day,0.5 hours per time,2 cigarettes per time,5 days per week,for 12 consecutive weeks;Each concentration of ginsenoside Rh2 was administered by oral gavage 1 hour before smoking in the group treated with ginsenoside Rh2 for pre intervention,with a dosage of 20 ml/kg,once every other day,three times a week,for 12 consecutive weeks.After the smoking was completed,the COPD model group and the Pumicole Lingshu group were nebulized for 3 minutes using an atomizer,once a day,for 15 consecutive days;The groups treated with ginsenoside Rh2 at different concentrations continued to be administered orally as mentioned above.Detection of lung dynamic compliance(Cdyn),functional residual volume(FRC),maximum expiratory volume(FVC),airway resistance(RI),mean alveolar number(MAN),mean alveolar intercept(MLI),and the content of smooth muscle actin α in vascular and bronchial walls(α-SMA),serum interleukin-1 β(IL-1β),Tumor necrosis factor-α(TNF-α)and the activity of superoxide dismutase(SOD).Results Compared with the blank control group,the COPD model group and the mice treated with various concentrations of ginsenoside Rh2 showed higher levels of Cdyn and RI in lung function,while lower levels of FRC and FVC.Except for the Cdyn,RI,and FRC in the group treated with 1 mg/ml ginsenoside Rh2,the differences were statistically significant(P＜0.05).Compared with the COPD model group,the lung function Cdyn and RI of mice exposed to different concentrations of ginsenoside Rh2 were lower,while FRC and FVC were higher.Except for the Cdyn group exposed to 0.5 mg/ml ginsenoside Rh2,the differences were statistically significant(P＜0.05).Moreover,as the concentration of ginsenoside Rh2 increased,the Cdyn and RI of mice showed a downward trend,while FRC and FVC showed an upward trend.Compared with the blank control group,the COPD model group and the mice treated with various concentrations of ginsenoside Rh2 showed a decrease in lung tissue MAN,while MLI increased.Except for the group treated with 1 mg/ml ginsenoside Rh2,the differences were statistically significant(P＜0.05).Compared with the COPD model group,the lung tissue MAN of mice exposed to different concentrations of ginsenoside Rh2 increased while MLI decreased.Except for the MLI of the group exposed to 0.5 mg/ml ginsenoside Rh2,the differences were statistically significant(P＜0.05).And as the concentration of ginsenoside Rh2 increases,the mouse MAN showed a downward trend,while the MLI showed an upward trend.Compared with the blank control group,the COPD model group,the Pumico Lingshu group,and the mice exposed to ginsenoside Rh2 at different concentrations showed smooth muscle in the pulmonary vascular wall and bronchial wall of the mice α-SMA and serum IL-1β,TNF-α contents were high,while the serum SOD activity was low.Except for α-SMA and serum SOD of the group treated with 1 mg/ml ginsenoside Rh2,the differences were statistically significant(P＜0.05).Compared with the COPD model group,the smooth muscle of the pulmonary vascular wall and bronchial wall in mice exposed to ginsenoside Rh2 at different concentrations α-SMA and serum IL-1β,TNF-α content were relatively low,while the serum SOD activity was high.Except for α-SMA and serum SOD of the group treated with 0.5 mg/ml ginsenoside Rh2,the differences were statistically significant(P＜0.05).And as the concentration of ginsenoside Rh2 increased,the smooth muscle of the pulmonary vascular wall and bronchial wall in mice α-SMA and serum IL-1β,TNF-α contents showed a decreasing trend,while the serum SOD activity showed an increasing trend.Conclusion Ginsenoside Rh2 can enhance the body's immune function,inhibit small blood vessel walls in the lungs,and smooth muscle in the bronchi of the lungs α-SMA protein expression improves alveolar structure and goblet cell count in mucosal epithelium,thereby improving lung function and alleviating lung injury in COPD model mice.
Chronic obstructive pulmonary diseaseGinsenoside Rh2Pulmonary function
万方数据
维普
