Determination of 24 elements in whole blood by direct dilution-inductively coupled plasma mass spectrometry
Objective To establish the determination of 24 elements in whole blood by direct dilution-inductively coupled plasma mass spectrometry.Methods Diluting the whole blood sample 20 times by 0.1％Triton X-100+0.5％nitric acid as diluent,inductively coupled plasma mass spectrometer with a high-flow nebulizer in collision reaction cell(KED)mode were used to measure 24 elements using Sc,Y,ln,Ge,Bi solutions as online internal standards.Results The regression equations showed a good linear relationship with r≥ 0.999 0,for Li,Be,V,Cr,Mn,Co,Ni,As,Se,Sr,Mo,Cd,Sn,Sb,Te,T1 in the range of 0.1-10.0 μg/L,Mg,Ca in the range of 0.05-5.0 mg/L,Al,Cu in the range of 5.0-60.0 μg/L,Fe in the range of 0.01-2.0 mg/L,Zn in the range of 10.0-500 μg/L,Ba,Pb in the range of 0.5-25.0 μg/L.The detection limits of method were 0.008 µg/L-0.007 mg/L.The quantification limits of method were 0.025 pg/L-0.023 mg/L.The recovery rates were 81.9％-110.0％;The intra-batch precisions were 0.8％-7.9％,and the inter-batch precisions were 1.3％-9.2％.The measurement results of quality control sample are all within the uncertainty range.Conclusion The method has the advantages of high sensitivity,simple pre-processing,easy operation,and wide linear range.The precision and accuracy meet the requirements for determination of biological sample,and can be used for biological monitoring of large batches of samples in the population and rapid screening of poisoned samples.
Whole bloodDirect dilutionInductively coupled plasma mass spectrometry
万方数据
维普
