Effect of oxaliplatin on transcription profile of human colon cancer cells
Objective To understand the effect of oxaliplatin(OXA)on the transcriptional expression profile of human colorectal cancer(SW620)cells.Methods The CCK-8 assay was used to observe the proliferation inhibition effect of OXA on SW620 cells at the doses of 0(control),4,8,16,32,64,100 and 128 mg/L respectively,exposure for 24 hours,and the IC50(Half maximal inhibitory concentration)value(64 mg/L)was calculated as the dose concentration for transcriptome sequencing.RNA-seq was used for transcriptome sequencing,differential gene expression was screened,and GO enrichment analysis,KEEG enrichment analysis,PPI network construction were performed to investigate the differential genes,signaling pathways,and biological processes possibly involved in the tumor proliferation inhibitory effect of OXA.Results Compared with the control group,the survival rates of SW620 cells exposed to 16,32,64,100 and 128 mg/L OXA all decreased significantly(P＜0.05),with a dose dependent manner.Compared with the control group,there were 8 187 differential expressed genes in the OXA group,including 2 114 upregulated genes and 6 073 downregulated genes.GO enrichment analysis results showed that differential expressed genes were mainly enriched in processes such as the Wnt signaling pathway,protein serine/threonine kinase activity,protein kinase activity,protein phosphorylation,etc.KEEG enrichment analysis results showed that differential expressed genes were mainly enriched in pathways such as adherens junction,endocytosis,proteoglycans in cancer,pathways in cancer,Wnt signaling pathway,etc.Through PPI network construction,MCODE and CytoHubba algorithms identified 10 hub genes,namely UQCRQ,NDUFA1,ATP51F1,UQCR11,COX6A 1,COX7A2,COX7B,NDUFA6,NDUFA4,COX8A.Conclusion The proliferation inhibitory effect of OXA on human colorectal cancer cells SW620 may be related to the regulation of the cell mitochondrial respiratory chain and oxidative phosphorylation.
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