Oxidative stress damage of octogen to DNA in V79 cells
Objective To investigate the cytotoxicity and DNA damage of octogen(HMX)on Chinese hamster lung fibroblasts(V79 cells).Methods Using in vitro cultured V79 cells as the research object,the cell proliferation and toxicity detection experimental method(CCK-8 method)was used to detect the toxic effects of 5 concentrations(5,25,50,75 and 100 mg/L)of HMX solution on V79 cells after 24 hours of exposure;Single cell gel electrophoresis(SCGE)and Elisa method were used to determine the DNA damage index and oxidative stress index of V79 cells exposed to HMX solution at three concentrations(50,75,100 mg/L)for 24 h.Results Compared with the solvent control group,in 50,75,and 100 mg/L groups,the survival rate of V79 cells significantly decreased(P＜0.05),and with the increase of HMX concentration,the survival rate of V79 cells showed a decreasing trend.Compared with the solvent control group,in 50-100 mg/L groups,the tail length,tail DNA％,tail distance,and live tail distance of V79 cells significantly increased(P＜0.05),and with the increase of HMX concentration,the tail length,tail DNA％,tail distance,and live tail distance of V79 cells all showed an upward trend.Compared to the solvent control group,in 75 and 100 mg/L groups,the 8-OHdG level in V79 cells significantly increased(P＜0.05),and with the increase of HMX concentration,the 8-OHdG level of V79 cells showed a trend of first increasing and then decreasing.Compared to the solvent control group,only in 100 mg/L group,the ROS level of V79 cells significantly increased(P＜0.05),and with the increase of HMX concentration,the ROS level of V79 cells showed an initial upward trend.Compared to the solvent control group,in 75 and 100 mg/L groups,the MDA level of V79 cells significantly increased(P＜0.05),and with the increase of HMX concentration,the MDA level of V79 cells showed an upward trend.Compared with the solvent control group,the GSH levels of V79 cells in the groups treated with HMX at different concentrations decreased significantly(P＜0.05),and with the increase of HMX concentration,the GSH level of V79 cells showed a downward trend.Compared to the solvent control group,only in 75 mg/L group,the SOD level of V79 cells significantly decreased(P＜0.05),and with the increase of HMX concentration,the SOD level of V79 cells showed a trend of first decreasing and then increasing.Conclusion In the present experiment,HMX can induce cytotoxicity by causing oxidative damage and significantly inhibit V79 cell proliferation,resulting in DNA damage.
万方数据
维普
