Rapid detection method of human urine elements by inductively coupled plasma mass spectrometry based on integrated injection system
Objective To establish a rapid method for the determination of 18 elements in urine by inductively coupled plasma mass spectrometry(ICP-MS).Methods The urine samples(400 μl)were added with 4 ml 2％nitric acid+100 μg/L Au solution.After pre-treatment,the samples were injected into the integrated injection system(ISIS).As the samples entered octopole reaction system,the interference was eliminated by kinetic energy discrimination(KED)using helium as it's collision gas.The contents of 18 elements in urine were determined by ICP-MS.Results This method was linear for all elements(correlation coefficient ≥0.99)in the range of 0.1-1 000.0 μg/L.All 18 elements in urine were detected with the limit of detection(LOD)ranging from 0.003 μg/L to 0.96 μg/L,limit of quantification(LOQ)ranging from 0.01 μg/L to 3.22 μg/L.The recoveries of 18 elements in urine ranged from 82.1％to 110.4％,and the RSDs ranged from 0.7％to 30.5％.In addition,the efficiency of testing was increased from 25 samples per hour to 52 samples per hour.Conclusion This method has the advantages of simple pre-treatment and rapid sample injection,and the sensitivity,precision,and repeatability meet the requirements of urine elements detection.Overall,the method is particularly useful for screening,cohort studies and other urine tests of large sample.
Inductively coupled plasma mass spectrometryUrineIntegrated injection systemElements
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维普
