Objective To develop an HPLC-MS-MS analytical method for simultaneous determination of amorofine,butenafine,sertaconazole,fibamzone,crotamiton and other five anti-infective drugs in cosmetics.Methods The sample was extracted with acetonitrile under ultrasonication,and filtered by PTEF filter membrane.The analytes were separated on Luna C8 column(100 mm×2 mm,3 μm),the column temperature was 30 aC and the flow rate was 0.3 ml/min.The mobile phase consisted of 0.5％formic acid acetonitrile-0.5％formic acid water.The compounds were identified under electrospray ionization and multiple reactions monitoring(MRM)mode.Results The linear ranges of amorofine were 0.004-0.2 μg/ml,that of butenafine and crotamiton were 0.002-0.1 μg/ml,those of sertaconazole and fibamzone were 0.02-1 μg/ml.In the ranges,the obtained regression equations all showed good linear correlation(r＞0.998).The LODs of five anti-infective drugs were 0.02-0.9 mg/kg.The recoveries of five anti-infective drugs in 3 different dosage forms of cosmetics ranged 87.3％-107.9％,with RSDs of 1.6％-13.4％.Conclusion This method is effective,simple and accurate,and it is applicable to the simultaneous determination of five anti-infective drugs in various cosmetics substrates in cosmetics.
维普
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