首页|水中苯并三唑类紫外吸收剂的分散固相萃取-高效液相色谱测定法

水中苯并三唑类紫外吸收剂的分散固相萃取-高效液相色谱测定法

Determination of ultraviolet benzotriazole absorbent in water by dispersive solid phase extraction and high performance liquid chromatography

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目的 建立水环境中4种苯并三唑类紫外吸收剂的高效液相色谱测定法.方法 取35 ml水样,调节水样pH值为6,采用40 mg Ce/MOF-801固相萃取柱富集5 min,1 ml乙腈洗脱待测物,经C18色谱柱(4.6 mm×250 mm,5 μm)分离,以乙腈∶水(体积比为52∶48)为流动相进行等度洗脱,柱温25 ℃.采用紫外检测器(200 nm)测定4种苯并三唑类紫外吸收剂,外标法定量.结果 在0.02～1.00 μg/ml的范围内,4种苯并三唑类紫外吸收剂所得回归方程的线性关系良好,r≥0.999 2.该方法的检出限为 0.057～0.117 μg/L,定量下限为 0.171～0.351 μg/L;平均回收率为 79.3％～107.2％,RSD 为 0.51％～4.47％.吸附剂可重复使用至少8次,富集因子可以达到56.6～73.9.结论 该方法具有灵敏、简便且快速等优点,适用于水环境中痕量4种紫外吸收剂的检测.
Objective To establish a method for the determination of four benzotriazole UV absorbers(BZTs)in aqueous environment through high performance liquid chromatographic(HPLC).Methods Totally 35 ml of water sample was collected and adjusted the pH value to 6.The targets were enriched for 5 minutes by a sorbent column loaded 40 mg of Ce/MOF-801 and eluted by 1 ml acetonitrile further.An C18(4.6 mm×250 mm,5 μm)column was used for HPLC separation.The mobile phase consisted of acetonitrile-water(52∶48,V/V),the temperature of the column was held constant at 25 ℃,and the four benzotriazole UV absorbers were determined by UV detector with wavelength of 200 nm.External standard method was used to quantify.Results The linearity of the four benzotriazoles UV absorbers was good in the range of 0.02-1.00 μg/mL withr 0.999 2.The limits of detection were 0.057-0.117 μg/L and the lower limits of quantification were 0.171-0.351 μg/L.The average recoveries were 79.3％-107.2％and the precision was 0.51％-4.47％.The adsorbent could be reused at least 8 times and the enrichment factor could reach 56.6-73.9.Conclusion The method is sensitive,simple and rapid,and is applicable to the determination of trace amounts of the four UV absorbers in the aqueous environment.

WaterBenzotriazolesMetal organic frameworkDispersive solid phase extractionChromatography,high performance liquid

李欣欣、贾叶青、李淼、王曼曼、王茜、郝玉兰

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华北理工大学公共卫生学院,河北唐山 063210

河北省煤矿卫生与安全重点实验室

天津市疾病预防控制中心卫生检验检测所理化科

水 苯并三唑类 金属有机骨架 分散固相萃取 色谱法,高效液相

国家自然科学基金华北理工大学公共卫生学院高水平科研创新团队建设计划大学生创新创业训练计划河北省煤矿卫生与安全重点实验室项目

82073608KYTD202302X2022309

2024

10.16241/j.cnki.1001-5914.2024.05.011

环境与健康杂志
中华预防医学会,天津市疾病预防控制中心

环境与健康杂志

CSTPCD
影响因子：0.658
ISSN：1001-5914
年,卷(期)：2024.41(5)